PERK Rabbit Monoclonal Antibody

Catalogue Numbers: MB66377-50, MB66377-100

Sizes: 50µl, 100µl

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: Q9NZJ5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition . ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress . PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain . Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of PERK protein.

Extra Notes: Western blot analysis of PERK expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.

Bioworld Molecular Weight: ~ 140 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

Immunogen: Recombinant protein of human PERK

Conjugate: Unconjugated

Modification: Unmodified