PARG Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71578-50, BS71578-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86W56

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:1000 - 1:2000

Background: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 111KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of PC-3 cells, using PARG antibody at 1:480 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Alternative Name: PARG; PARG99

Immunogen: Recombinant fusion protein of human PARG(NP_003622.2).

Conjugate: Unconjugated

Modification: Unmodified