Phospho-(Ser/Thr) ATM/ATR Substrate Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79460-50, BS79460-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 38-68KDa

Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S &T.

Conjugate: Unconjugated

Modification: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho- ATM/ATR Substrate pAb at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.