PDCD4 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS91038-50, BS91038-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q53EL6(Human) Q61823(Mouse) Host: Rabbit Reactivity: Human, Mouse Applications: WB, ICC IHC FC All Applications: WB:1:500 ICC:1:20-1:50 IHC:1:50-1:200 FC:1:50-1:100 Background: The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the co-activator p300 by c-Jun.  Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: PDCD4 polyclonal antibody detects endogenous levels of PDCD4 protein. Bioworld Molecular Weight: 52 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: ICC staining PDCD4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining PDCD4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alternative Name: PDCD4, H731, Death up-regulated gene protein, Dug, H731, Ma3, MGC33046, MGC33047, Neoplastic transformation inhibitor, Neoplastic transformation inhibitor protein, Nuclear antigen H731, Nuclear antigen H731 like, Nuclear antigen H731 like protein, Nuclear antigen H731-like, PDCD 4, Pdcd4, PDCD4, Programmed cell death 4, programmed cell death 4 (neoplastic transformation inhibitor), Programmed cell death protein 4, Protein 197/15a, Protein MA-3, Tis, Topoisomerase-inhibitor suppressed protein, Immunogen: Recombinant protein Conjugate: Unconjugated Modification: Unmodified