SARS-CoV-2 3CLpro Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80245-50, BS80245-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0DTC2

Host: Rabbit

Reactivity: SARS-CoV-2

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: [Replicase polyprotein 1ab]: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 30-35KDa

Immunogen: Recombinant fusion protein of coronavirus 3CLpro(YP_009725295.1).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CL pro fusion protein and untreated 293T cells use SARS-CoV-2 3CLpro Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.