Oct-3/4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90981-50, BS90981-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01860(Human) P20263(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF IHC, IP, CHIP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, OTF3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Oct-3/4 polyclonal antibody detects endogenous levels of Oct-3/4 protein.

Bioworld Molecular Weight: 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: F9
Lane 2: NCCIT
Lane 3: hES, ICC staining Oct4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Oct-4, Oct-3, OTF-3, POU5F1, OCT3, OCT4, OTF3, Octamer binding transcription factor 4, MGC22487, Oct 3, Oct 4, Oct-3, Oct-4, OCT3, Oct4, Octamer binding protein 3, Octamer binding protein 4, Octamer binding transcription factor 3, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, OTF 3, OTF 4, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain class 5 transcription factor 1, POU domain transcription factor OCT4, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified