RAB4A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0161-50, MB0161-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20338

Host: Mouse

Reactivity: H, M, R

Applications: WB ICC

All Applications: WB: 1:1000
ICC: 1:100~300

Background: The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of RAB4A and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size:24KDa
Observed band size:30KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western BlotDetection of RAB4A in Rat stomach, Mouse brain, Hela & MCF7 cell lysates and using RAB4A antibody (1:1000 diluted).

Alternative Name: Ras-related protein Rab-4A; RAB4

Immunogen: Recombinant full length Human RAB4A.

Conjugate: Unconjugated

Modification: Unmodified