PTCD1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76689-50, BS76689-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75127

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a mitochondrial protein that binds leucine tRNAs and other mitochondrial RNAs and plays a role in the regulation of translation. Increased expression of this gene results in decreased mitochondrial leucine tRNA levels. Naturally occurring read-through transcription exists between upstream ATP5J2 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2) and this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 79kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PTCD1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of C6 cells using PTCD1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using PTCD1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using PTCD1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PTCD1

Immunogen: Recombinant fusion protein of human PTCD1(NP_056360.2).

Conjugate: Unconjugated

Modification: Unmodified