Progesterone Receptor Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91114-50, BS91114-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06401(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor, PR-A and PR-B, which are transcribed from distinct, estrogen-inducible promoters within a single copy of the PR gene. The first 164 amino acids of PR-B are absent in PR-A. Progesterone-bound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity. An inhibitory domain (ID), which maps to the amino terminus of the receptor, exists within both PR isoforms. Interestingly, the ID is functionally active only in PR-A and is necessary for steroid hormone transrepression by PR-A, suggesting that PR-A and PR-B may have different conformations in the cell. Phosphorylation of human PR occurs on at least nine serine residues. Phosphorylation of three of the residues is hormone-inducible (Ser 102, Ser 294 and Ser 345); the others are basal but hormone-stimulated.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Progesterone Receptor polyclonal antibody detects endogenous levels of Progesterone Receptor protein.

Bioworld Molecular Weight: 99 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Progesterone Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Progesterone Receptor antibody. Counter stained with hematoxylin.

Alternative Name: PR, PGR, NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, PRGR, Progesterone receptor, Progestin receptor form A, Progestin receptor form B,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified