CD276 Recombinant Protein

Catalogue Number: NCP0197-500, NCP0197-1000

Sizes: 500ug, 1mg

Swiss-Prot: Q5ZPR3

Host: E.coli

Tag: His-tag

AA Sequence: CTGGAAGTGCAGGTTCCGGAAGATCCGGTTGTTGCACTGGTTGGTACCGATGCAACCCTGTGTTGCAGCTTCAGTCCGGAACCGGGCTTCAGCCTGGCCCAGCTGAATCTGATCTGGCAGCTGACCGATACCAAACAGCTGGTTCATAGCTTCGCAGAAGGTCAGGATCAGGGCAGCGCATACGCTAATCGCACCGCCCTGTTCCCGGATCTGCTGGCACAGGGCAATGCCAGTCTGCGTCTGCAGCGCGTGCGCGTGGCCGATGAAGGCAGCTTCACCTGCTTCGTTAGCATTCGCGACTTCGGTAGTGCAGCAGTGAGCCTGCAGGTTGCCGCCCCGTATAGTAAACCGAGCATGACCCTGGAACCGAATAAAGATCTGCGTCCGGGCGATACCGTTACCATTACCTGCAGTAGTTATCAGGGTTATCCGGAAGCCGAAGTGTTCTGGCAGGATGGCCAGGGCGTTCCGCTGACCGGCAATGTTACCACCAGTCAGATGGCAAATGAACAGGGCCTGTTCGATGTTCATAGCATTCTGCGTGTGGTGCTGGGCGCAAATGGTACCTATAGTTGCCTGGTGCGTAATCCGGTGCTGCAGCAGGATGCACATAGCAGTGTGACCATTACCCCGCAGCGTAGTCCGACCGGCGCAGTTGAAGTGCAGGTGCCGGAAGATCCTGTGGTTGCCCTGGTTGGTACAGATGCAACCTTACGCTGCAGCTTCAGCCCGGAACCGGGCTTCAGTCTGGCCCAGTTAAATCTGATCTGGCAACTGACCGATACAAAACAGCTGGTGCATAGCTTCACCGAAGGTCGCGATCAGGGCTCAGCCTATGCCAATCGTACCGCCCTGTTCCCTGATCTGCTGGCGCAGGGCAATGCGAGTCTGCGTCTTCAGCGCGTGCGTGTGGCAGATGAAGGTAGCTTCACCTGCTTCGTGAGTATTCGCGACTTCGGCAGTGCAGCCGTTAGTCTGCAGGTGGCAGCACCGTATAGCAAACCGAGTATGACCCTGGAGCCGAATAAAGACCTGCGCCCGGGCGATACAGTGACCATTACATGCAGCAGTTATCGCGGCTATCCGGAAGCGGAAGTGTTCTGGCAAGATGGCCAGGGTGTGCCGCTGACCGGTAATGTTACCACAAGTCAGATGGCCAATGAACAGGGTCTGTTCGATGTGCATAGTGTTCTGCGTGTGGTTCTGGGCGCCAATGGTACCTACAGCTGCCTGGTGCGCAATCCGGTTCTGCAGCAGGACGCCCATGGTAGTGTTACCATTACAGGTCAGCCGATGACCTTCCCGCCGGAAGCC

Restriction Sites: NdeI-XhoI

Background: B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain. Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response. Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation. One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~48kDa

Notes: For research use only, not for use in diagnostic procedure.