Recombinant Human FTL Protein (His Tag)

Size: 50μg

Catalogue Number: PKSH033328-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: FTL

Target Symptom: Ferritin L subunit; Ferritin light chain; FTL

Research Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism;

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-6His

UNIProt ID: P02792

Accession: P02792

Background: Ferritin is a large; iron-storage heteropolymeric protein; which is expressed in most kinds of cells and co-assemble in different proportion in a tissue-specific manner. Ferritin has oligomer of 24 subunits and two types of subunits including light chain(FTL) and heavy chain. Ferritin can remove Fe (Ⅱ) from solution in the presence of oxygen and is very important for iron homeostasis. Iron is absorbed in the ferrous form and deposited as ferric hydroxides after oxidation. Iron is first oxidized to the ferric state for storage as ferric oxyhdroxide whithin the protein shell of ferritin. Thus; ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.

Sequence: Met 1-Asp175

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris; 250mM NaCl; 1mM EDTA; pH9.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 24.5 kDa

Observed MW: 20-25 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only