MAFK Monoclonal Antibody

Catalogue Numbers: MB66733-50, MB66733-100

Sizes: 50µl, 100µl

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O60675

Host: Mouse

Reactivity: Human

Applications: WB, FC

All Applications: WB (1/500 - 1/1000), FC (1/10 - 1/50)

Background: Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter.c-Maf (Maf-2) and the closely related family members Neural retina leucine zipper (Nrl), L-Maf and Krml1/MafB (Maf-1) all bind to T-MARE sites and are implicated in a wide variety of developmental and physiologic roles. The three small Maf family proteins MafF, MafG and MafK are components of NF-E2 which function as heterodimers with the large tissue restricted subunit of NF-E2 called p45, and furthermore are implicated in the transcriptional regulation of many erythroid-specific genes. MafG is ubiquitously expressed, with highest expression in the VMS, heart and skeletal muscle; its expression is induced in response to stress. MafK, also designated NF-E2 p18, is primarily expressed during development in mesenchymal and hematopoietic cells and neurons. MafK heterodimerizes with NF-E2 and various CNC proteins. MafF is most abundantly expressed in the lung and is thought to compensate for loss of function mutations in MafG and MafK.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MAFK protein.

Extra Notes: Western blot analysis of MAFK expression in Jurkat (A) whole cell lysates.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit

Immunogen: KLH-conjugated synthetic peptide of human MAFK. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified