FEN1 Monoclonal Antibody

Catalogue Numbers: MB66689-50, MB66689-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P39748

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IP

All Applications: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background: Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways. The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers. FEN-1 is also required for non-homologous end joining of double-stranded DNA breaks in long patch base excision repair. The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions, post-translational modifications, and subcellular re-localization in response to cell cycle or DNA damage.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of FEN1 protein.

Extra Notes: Western blot analysis of FEN1 expression in Hela (A), Jurkat (B), NIH3T3 (C), C6 (D), CEM (E), Ramos (F) whole cell lysates.

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1

Immunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified