Brachyury Monoclonal Antibody

Catalogue Numbers: MB66718-50, MB66718-100

Sizes: 50µl, 100µl

Product: Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O15178

Host: Mouse

Reactivity: Human, Mouse

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Brachyury protein, encoded by the T gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis. In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die in utero and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois. Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors. A common polymorphism in the human T gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Brachyury protein.

Extra Notes: Western blot analysis of Brachyury expression in mouse kidney (A) whole cell lysates.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Brachyury protein; Protein T

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Brachyury. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified