p95 NBS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91011-50, BS91011-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60934(Human) Q9R207(Mouse) Q9JIL9(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, FC IHC CHIP,

All Applications: WB:1:1,000
ICC:1:50-1:200
FC:1:50-1:100
IHC:1:50

Background: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: p95 NBS1 polyclonal antibody detects endogenous levels of p95 NBS1 protein.

Bioworld Molecular Weight: 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Nibrin on human lung lysates using anti-p95 NBS1 antibody at 1/1,000 dilution., ICC staining p95 NBS1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NBN, AT V1, AT V2, At V, Cell cycle regulatory protein p95, FLJ10155, MGC87362, Nbn, NBN, NBS 1, NBS, NBS1, Nibrin, Nijmegen breakage syndrome 1 (nibrin), Nijmegen breakage syndrome, Nijmegen breakage syndrome protein 1, p95, p95 protein of the MRE11/RAD50 complex,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified